Regulatory

Part:BBa_I741018:Experience

Designed by: Lucien Weiss   Group: iGEM07_Penn_State   (2007-06-21)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I741018

Description: The xylose F promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included as well as a CRP activator binding site.

Related parts:

  • BBa_I741022 CRP open reading frame
  • BBa_I741023 CRP* open reading frame (modified version of CRP which activates without being bound to cAMP)
  • BBa_I741005 xylR ORF

If you are having problems cloning with this part consider using one of these promoters:

  • BBa_I741015 Two way promoter controlled by xylR-xylose and CRP-cAMP
  • BBa_I741017 Two way promoter controlled by xylR-xylose and CRP-cAMP (reverse of BBa_I741015)
  • BBa_I741018 Right facing promoter (for xylF) controlled by xylR and CRP-cAMP
  • BBa_I741019 Right facing promoter (for xylA) controlled by xylR and CRP-cAMP
  • BBa_I741020 Right facing promoter (for xylF) controlled by xylR, with CRP binding site removed
  • BBa_I741021 Right facing promoter (for xylA) controlled by xylR, with CRP binding site removed

Promoter halfsites being bound by XylR (modeled by George Khoury PSU iGEM 2007)

User Reviews

UNIQd2d4aa3d37280a06-partinfo-0000000B-QINU UNIQd2d4aa3d37280a06-partinfo-0000000C-QINU